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Literature summary extracted from

  • Olsen, O.H.; Persson, E.
    Cofactor-induced and mutational activity enhancement of coagulation factor VIIa (2008), Cell. Mol. Life Sci., 65, 953-963.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.21 additional information enzyme activation involves residue 343, mechanisms, overview Homo sapiens
3.4.21.21 tissue factor TF TF-induced allosteric activation of FVIIa, binding structure, overview Homo sapiens
3.4.21.21 vitamin K dependent on Homo sapiens

Application

EC Number Application Comment Organism
3.4.21.21 medicine recombinant FVIIa constitutes an efficient substitution therapy for treatment of patients with haemophilia A and haemophilia B which lack or have dysfunctional FVIII and FIX, respectively, pharmacological mechanism of action of FVIIa, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.21 K337A site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
3.4.21.21 additional information construction of FVIIa analogues with a stabilized activation domain and N-terminal insertion Homo sapiens
3.4.21.21 P10Q site-directed mutagenesis, the mutant shows 2fold enhancement in membrane binding affinity over wild-type FVIIa Homo sapiens
3.4.21.21 P10Q/K32E site-directed mutagenesis, the mutant shows 27fold enhancement in membrane binding affinity over wild-type FVIIa, the double mutant displays a significantly improved procoagulant effect in haemophilic blood Homo sapiens
3.4.21.21 P10Q/K32E/D33F/A34E site-directed mutagenesis, the mutant shows 150-300fold enhancement in membrane binding affinity over wild-type FVIIa Homo sapiens
3.4.21.21 V158D/E296V/M298Q site-directed mutagenesis, FVIIa analogues with a stabilized activation domain and N-terminal insertion, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.21 extracellular
-
 Homo sapiens
-
-
3.4.21.21 membrane the major function of the enzyme's Gla domain is to attach FVIIa to membranes, i.e. to add binding energy to the interaction with membrane-associated tissue factor Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.21 Ca2+ activates Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.21 factor IX + H2O Homo sapiens
-
 factor IXa + ?
-
 ?
3.4.21.21 Factor X + H2O Homo sapiens
-
 Factor Xa + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.21 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.21 proteolytic modification zymogen FVII is converted to the activated form by proteolytic cleavage of the Arg152{15}-Ile153{16} peptide bond, zymogen factor VII is activated by thrombin Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.21 factor IX + H2O
-
 Homo sapiens factor IXa + ?
-
 ?
3.4.21.21 Factor X + H2O
-
 Homo sapiens Factor Xa + ?
-
 ?
3.4.21.21 additional information structure-function relationship, the enzyme forms a complex with the cell surface receptor tissue factor TF required for attaining its catalytically competent conformation, Asp338 is a catalytically important residue Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.21 More structure-function relationship, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.21 FVIIa
-
 Homo sapiens
3.4.21.21 More the enzyme is a member of the trypsin family of serine proteases Homo sapiens